Evaluation of an alternative extraction procedure for enterotoxin determination in dairy products
| Autor: | V. Atrache, C. Bavai, A. Meyrand, M.P. Montet, C. Vernozy-Rozand |
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| Rok vydání: | 1999 |
| Předmět: |
Staphylococcus aureus
Chromatography Chemistry Staphylococcal Enterotoxins Extraction (chemistry) Pasteurization Enterotoxin Applied Microbiology and Biotechnology Staphylococcal Food Poisoning law.invention chemistry.chemical_compound Enterotoxins Biochemistry law Cheese Animals Chemical Precipitation Extraction methods Trichloroacetic acid Trichloroacetic Acid Dialysis |
| Zdroj: | Letters in applied microbiology. 28(6) |
| ISSN: | 0266-8254 |
| Popis: | A concentration protocol based on trichloroacetic acid precipitation was evaluated and compared with the reference method using dialysis concentration. Different quantities of purified staphylococcal enterotoxins were added to pasteurized Camembert-type cheeses. Detection of enterotoxins in these cheeses was performed using an automated detection system. Raw goat milk Camembert-type cheeses involved in a staphylococcal food poisoning were also tested. Both enterotoxin extraction methods allowed detection of the lowest enterotoxin concentration level used in this study (0.5 ng g -1 ). Compared with the dialysis concentration method, TCA precipitation of staphylococcal enterotoxins was user-friendly' and less time-consuming. These results suggest that TCA precipitation is a rapid (1 h), simple and reliable method of extracting enterotoxin from food which gives excellent recovery from dairy products. |
| Databáze: | OpenAIRE |
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