Development and Characterization of an Allergic Asthma Rat Model for Interventional Studies

Autor: Margarida Castell, Ivan Best, Malén Massot-Cladera, María J. Rodríguez-Lagunas, Trinitat Cambras, Marta Périz, Francisco J. Pérez-Cano, Santiago Pastor-Soplin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Hypersensitivity
Immediate
Fisiologia patològica
Immunoglobulin E
Motor activity
lcsh:Chemistry
Temperatura corporal
Body temperature
Lung
Pathological physiology
lcsh:QH301-705.5
Spectroscopy
Rates (Animals de laboratori)
Leukotriene
bronchoalveolar lavage fluid
medicine.diagnostic_test
biology
Intranasal challenge
Bronchoalveolar lavage fluid
leukotriene
General Medicine
respiratory system
Computer Science Applications
purl.org/pe-repo/ocde/ford#3.02.27 [http]
Shock (circulatory)
Cytokines
Female
Brown Norway rats
IgE
medicine.symptom
Antibody
Leukotrienes
intranasal challenge
Ovalbumin
Rats as laboratory animals
Motility
Article
Catalysis
Inorganic Chemistry
Hypersensitivity
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
Anaphylaxis
Administration
Intranasal
Asma
motor activity
business.industry
Organic Chemistry
Epithelial Cells
Allergens
Anafilaxi
Asthma
Rats
respiratory tract diseases
Disease Models
Animal
Bronchoalveolar lavage
lcsh:Biology (General)
lcsh:QD1-999
Immunology
biology.protein
Nasal administration
business
body temperature
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 3841, p 3841 (2020)
Dipòsit Digital de la UB
Universidad de Barcelona
International Journal of Molecular Sciences
Volume 21
Issue 11
CONCYTEC-Institucional
Consejo Nacional de Ciencia Tecnología e Innovación Tecnológica
instacron:CONCYTEC
ISSN: 1661-6596
1422-0067
Popis: Allergic asthma is one of the most common chronic diseases of the airways, however it still remains underdiagnosed and hence undertreated. Therefore, an allergic asthma rat model would be useful to be applied in future therapeutic strategy studies. The aim of the present study was to develop an objective model of allergic asthma in atopic rats that allows the induction and quantification of anaphylactic shock with quantitative variables. Female Brown Norway rats were intraperitoneally sensitized with ovalbumin (OVA), alum and Bordetella pertussis toxin and boosted a week later with OVA in alum. At day 28, all rats received an intranasal challenge with OVA. Anaphylactic response was accurately assessed by changes in motor activity and body temperature. Leukotriene concentration was determined in the bronchoalveolar lavage fluid (BALF), and total and IgE anti-OVA antibodies were quantified in blood and BALF samples. The asthmatic animals&rsquo
motility and body temperature were reduced after the shock for at least 20 h. The asthmatic animals developed anti-OVA IgE antibodies both in BALF and in serum. These results show an effective and relatively rapid model of allergic asthma in female Brown Norway rats that allows the quantification of the anaphylactic response.
Databáze: OpenAIRE
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