Reactivity of isolated human chorionic vessels: analysis of some influencing variables
| Autor: | Francisco J. Morales-Olivas, Vicente Serra, Antonio Abad, L. Estañ |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
medicine.medical_specialty Adolescent Physiology Placenta Pregnancy Trimester Third Chorionic vessels Cold storage In Vitro Techniques Biology Potassium Chloride Veins Pregnancy Refrigeration Physiology (medical) Internal medicine Reaction Time medicine Humans reproductive and urinary physiology Pharmacology Cesarean Section Gestational age Arteries Chorion General Medicine Delivery Obstetric medicine.anatomical_structure Endocrinology Vagina embryonic structures Circulatory system Chorionic villi Female medicine.symptom Vasoconstriction Blood vessel |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 81:1147-1151 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/y03-119 |
| Popis: | The aim of the study was to determine whether 24 h of cold storage of samples, mode of delivery, and gestational age influenced in vitro human chorionic vascular reactivity (35 arteries and 34 veins). The following groups were compared: (i) fresh versus 24-h cold-stored (4 °C in Krebs–Henseleit solution) chorionic vascular rings from normal term placentas, (ii) fresh chorionic vascular rings from normal term placentas obtained after vaginal delivery versus those obtained after elective caesarean section, and (iii) fresh chorionic vascular rings from normal term placentas versus those obtained from preterm deliveries. Isometric recording of the concentration–response curve to KCl (5–120 mM) was assesed in each group. In vitro human chorionic vascular reactivity was influenced negatively by the 24-h cold storage of samples, with only 30% of stored samples being weakly reactive to KCl. Human chorionic vascular reactivity to KCl was unaffected by the mode of delivery. However, the response to KCl was gestational-age dependent. Thus, preterm vascular rings exhibited a significantly (P < 0.05) decreased response (Emax = 9.8 ± 0.0 mN; EC50 = 26.0 ± 1.3 mM) compared with term samples (Emax = 21.6 ± 2 mN; EC50 = 13.9 ± 1.6 mM). In conclusion, this study provides evidence that fresh term vascular rings are the tissues of choice for studying human chorionic vascular reactivity.Key words: human chorionic vessels, placenta, vascular reactivity. |
| Databáze: | OpenAIRE |
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