Endothelial cell‐derived fibroblast growth factor‐18 regulates ovarian function in sheep

Autor: Anthony Estienne, Lauriane Relav, Morad Benkoura, Danielle Monniaux, Fanny Morin, Stéphane Fabre, Christopher A Price
Rok vydání: 2022
Předmět:
Zdroj: Journal of Cellular Physiology. 237:2528-2538
ISSN: 1097-4652
0021-9541
DOI: 10.1002/jcp.30718
Popis: Increasing the efficiency of farm animal reproduction is necessary to reduce the environmental impact of food production systems. One approach is to increase the number of healthy eggs (oocytes) produced per female for fertilization, thus it is important to understand factors that decrease oocyte health. One paracrine factor that decreases ovarian follicle growth is fibroblast growth factor 18 (FGF18) secreted by cells in the theca layer of the ovarian follicle, however the factors that regulate FGF18 secretion are unknown. In this study we hypothesized that FGF18 secretion is controled by intrafollicular factors and is linked to fertility, which we tested by using cell culture and sheep genetic models in vivo. Separation of theca cell populations revealed that FGF18 messenger RNA (mRNA) is located mainly in thecal endothelial rather than endocrine cells, and immunohistochemistry localized FGF18 protein to microvessels in the theca layer in situ. Culture of ovine theca-derived endothelial cells was used to demonstrate stimulation of FGF18 mRNA and protein abundance by bone morphogenetic protein 4 (BMP4), a growth factor derived from theca endocrine cells. Taking advantage of a sheep genetic model, we demonstrate reduced ovarian and peripheral FGF18 concentrations in the hyperprolific Booroola ewe harboring the FecB
Databáze: OpenAIRE
Externí odkaz: