Two-stage model of chemically induced hepatocellular carcinoma in mouse
| Autor: | Yu-An Xie, Xiao-Ling Luo, Yuan-dong Li, Fan Yang, Sheng-xin Huang, Zhi-Peng Kuang, Ji-Ning Wu, Min Luo |
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| Rok vydání: | 2013 |
| Předmět: |
Oncology
Male Cancer Research medicine.medical_specialty Programmed cell death Cirrhosis Carcinoma Hepatocellular Tumor suppressor gene Carcinogenesis CCL4 Apoptosis Biology Proto-Oncogene Proteins c-myc Mice Internal medicine medicine Carcinoma Animals Humans Diethylnitrosamine Carbon Tetrachloride Cells Cultured Cell Proliferation Monomeric GTP-Binding Proteins Regulation of gene expression Mice Inbred BALB C Liver Neoplasms General Medicine medicine.disease digestive system diseases Gene Expression Regulation Neoplastic Disease Models Animal Liver Hepatocellular carcinoma Cancer research Hepatocytes Tumor Suppressor Protein p53 |
| Zdroj: | Oncology research. 20(11) |
| ISSN: | 1555-3906 |
| Popis: | The aim of this study was to develop an efficient and reproducible mouse model for hepatocellular carcinoma (HCC) research and assess the expression of two proto-oncogenes (c-myc and N-ras) and tumor suppressor gene p53 in the carcinogenic process. In this study, we found that diethylnitrosamine initiation with CCl4 and ethanol promotion could induce a short-term, two-stage liver carcinogenesis model in male BALB/c mice, the process of hepatocarcinogenesis including liver damage, liver necrosis/cell death, liver inflammation, liver proliferation, liver hyperplasia, liver steatosis, and liver cirrhosis and hepatocellular nodules, which mimicked the usual sequence of events observed in human HCC. We also identified that the increase in expression of the p53 gene is related to the proliferation of hepatocytes, whereas overexpression of the c-myc and N-ras genes is associated with hepatocarcinogenesis. This animal model may serve as a basis for recapitulating the molecular pathogenesis of HCC seen in humans. |
| Databáze: | OpenAIRE |
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