Photoreceptor-Specific Expression of Platelet-Derived Growth Factor-B Results in Traction Retinal Detachment
| Autor: | Nancy L. Derevjanik, Peter A. Campochiaro, Donald J. Zack, William J. LaRochelle, Haruhiko Yamada, Sean F. Hackett, Naoyuki Okamoto, Man Seong Seo, Melissa A. Vinores, Eri Yamada, Stanley A. Vinores |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Cell type Pathology medicine.medical_specialty Platelet-derived growth factor genetic structures medicine.medical_treatment Mice Transgenic Pathology and Forensic Medicine Mice chemistry.chemical_compound Glial Fibrillary Acidic Protein medicine Animals Humans RNA Messenger Platelet-Derived Growth Factor Retina biology Reverse Transcriptase Polymerase Chain Reaction Growth factor Retinal Detachment Retinal detachment Retinal Proto-Oncogene Proteins c-sis Blotting Northern medicine.disease eye diseases medicine.anatomical_structure chemistry Gliosis Fluorescent Antibody Technique Direct Astrocytes biology.protein Cattle Female Endothelium Vascular sense organs medicine.symptom Pericytes Platelet-derived growth factor receptor Regular Articles Photoreceptor Cells Vertebrate |
| Zdroj: | The American Journal of Pathology. 157:995-1005 |
| ISSN: | 0002-9440 |
| DOI: | 10.1016/s0002-9440(10)64612-3 |
| Popis: | Expression of platelet-derived growth factor (PDGF)-A and PDGF-B is increased in patients with proliferative retinopathies in which traction retinal detachments occur. Previous studies have demonstrated that increased expression of PDGF-A in the retina of transgenic mice results in retinal gliosis due to proliferation of astrocytes with different retinal phenotypes based on the time of onset and location of the PDGF-A production. In this study, we investigated the effects of PDGF-B in the retina using gain-of-function transgenic mice that express PDGF-B in photoreceptors. These mice show proliferation of astrocytes, pericytes, and, to a lesser extent, endothelial cells, resulting in ectopic cells on the surface and extending into the retina. The sheets of cells exert traction on the retina resulting in traction retinal detachments similar to those seen in humans with proliferative retinopathies. These studies suggest that PDGF-B has more dramatic effects in the retina than PDGF-A, because it acts on additional cell types, in particular on pericytes, which have a highly developed contractile apparatus. These studies in the retina suggest a means that could be used in other tissues throughout the body to achieve graded PDGF effects. They also provide a new model of traction retinal detachment that can be used to investigate new treatments for patients with proliferative retinopathies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|