Lysyl oxidase-like 2 expression is increased in colon and esophageal tumors and associated with less differentiated colon tumors
| Autor: | Peter Hollosi, Lloyd Asuncion, Sheri F. T. Fong, Keith S. K. Fong, E. Dietzsch, Katalin Csiszar, M. Iqbal Parker, Qingping He |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Cancer Research Stromal cell Esophageal Neoplasms Blotting Western Loss of Heterozygosity Lysyl oxidase Biology Decitabine Cell Line Loss of heterozygosity Genetics medicine Humans Promoter Regions Genetic Aged DNA Primers Regulation of gene expression Aged 80 and over LOXL2 Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation Anatomy Esophageal cancer Middle Aged medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic Tumor progression Cancer cell Colonic Neoplasms Cancer research Azacitidine CpG Islands Female Amino Acid Oxidoreductases |
| Zdroj: | Genes, chromosomescancer. 46(7) |
| ISSN: | 1045-2257 |
| Popis: | Lysyl oxidase-like 2 (LOXL2) belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. We previously demonstrated the association between increased LOXL2 expression and invasive/metastatic behavior in human breast cancer cells and mouse squamous and spindle cell carcinomas, interaction between LOXL2 and SNAIL in epithelial-mesenchymal transition, and localization of the LOXL2 gene to 8p21.2-21.3, within a minimally deleted region in several cancers, including colon and esophagus. In the present study, we analyzed LOXL2 expression in colon and esophageal tumors, and explored methylation as a regulator of LOXL2 expression. Immunohistochemistry using normal tissues demonstrated intracellular localization of LOXL2 in colonic enteroendocrine cells and esophageal squamous cells at the luminal surface, but not in mitotically active cells. Tissue array analysis of 52 colon adenocarcinomas and 50 esophageal squamous cell carcinomas revealed presence of LOXL2 expression in 83 and 92% of the samples, respectively, and a significant association between increased number of LOXL2-expressing cells and less-differentiated colon carcinomas. We determined that the methylation status of the 1150 bp 5' CpG island may contribute to the regulation of the gene. Loss of heterozygosity studies, using a microsatellite within intron 4 of the LOXL2 gene, revealed that loss of LOXL2 was unlikely to play a major role in either colon or esophageal tumors. These results suggest that increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. |
| Databáze: | OpenAIRE |
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