The pho regulon of Shigella flexneri
| Autor: | Jan Tommassen, Joost van Strien, Riny Janssen, Monica Scholten, Corné Bogaarts |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
inorganic chemicals
Recombinant Fusion Proteins Molecular Sequence Data Porins Sequence Homology medicine.disease_cause Regulon Microbiology Phosphates Shigella flexneri Plasmid Bacterial Proteins Species Specificity Escherichia coli medicine Amino Acid Sequence Molecular Biology Peptide sequence Base Sequence biology Escherichia coli Proteins Membrane Proteins Biological Transport Gene Expression Regulation Bacterial Phosphate-Binding Proteins Alkaline Phosphatase biology.organism_classification Biochemistry Genes Bacterial Periplasmic Binding Proteins Porin Mutagenesis Site-Directed bacteria Alkaline phosphatase Carrier Proteins Bacterial outer membrane Protein Kinases Sequence Alignment Transcription Factors |
| Zdroj: | Molecular Microbiology. 15:247-254 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1995.tb02239.x |
| Popis: | Growth of Escherichia coli K-12 in low-phosphate conditions results in the induction of the synthesis of many proteins, including the outer membrane porin PhoE, alkaline phosphatase, and the Pst system for the transport of phosphate (P1). This response is controlled by a two-component regulatory system of which PhoB and PhoR are the response-regulator and the sensor/kinase, respectively. When Shigella flexneri was starved for P1, neither PhoE nor alkaline phosphatase was produced. However, induction of the synthesis of the PstS protein was observed, indicating that S. flexneri contains a functional PhoB/PhoR regulatory system. Consistent with this notion, the introduction of the E. coli phoA gene in S. flexneri resulted in the induction of alkaline phosphatase synthesis under phosphate limitation. However, introduction of phoE on a plasmid did not lead to the expression of PhoE protein, indicating that S. flexneri PhoB does not recognize the phoE promoter region. The phoB gene was cloned and sequenced and in the deduced amino acid sequence two deviations from that of E. coli PhoB were detected. Site-directed mutagenesis revealed that one of these deviations, i.e. Leu-172, which is Arg in E. coli PhoB, is responsible for the lack of expression of the PhoE protein in S. flexneri. |
| Databáze: | OpenAIRE |
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