Identification and characterisation of a novel splice variant of synaptojanin1
| Autor: | Peter J. Parker, Peter M. Finan, Elisabeth Radley, Rüdiger Woscholski |
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| Rok vydání: | 1998 |
| Předmět: |
Molecular Sequence Data
Biophysics Constitutively active Nerve Tissue Proteins Computational biology Synaptojanin Biology Biochemistry Domain (software engineering) 5-Phosphatase Structural Biology Genetics Animals Amino Acid Sequence Cloning Molecular Proline rich Molecular Biology Base Sequence Alternative splicing Genetic Variation Synaptojanin1 Sequence Analysis DNA Cell Biology Lipid Rat brain Phosphoric Monoester Hydrolases Rats Alternative Splicing Identification (biology) Inositol Splice variant |
| Zdroj: | FEBS Letters. 432:5-8 |
| ISSN: | 0014-5793 |
| Popis: | Synaptojanin1, the major constitutively active PtdInsP3 5-phosphatase activity in rat brain, is one of two closely related proteins both extensively spliced in their C-terminal proline rich domain. We describe here the discovery of a novel splice variant of synaptojanin1 which misses the major N-terminal part of the SAC1 domain. This ΔSAC-synaptojanin1 is expressed in rat brain tissue as shown by Northern and Western analysis. However, the deletion of the SAC1 domain does not alter PtdInsP3 5-phosphatase activity demonstrating that the SAC1 domain is not necessary for catalytic function. |
| Databáze: | OpenAIRE |
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