Molecular characterization and pathological identification of a novel strain of delta papillomavirus-4 (bovine papillomavirus-2) in Egypt
| Autor: | Ahmed F. Afify, Momtaz A. Shahein, Rabab T. Hassanien, Gehan Shehab, Ali M. M. Abdelhakim, Mervat E. Hamdy, Mervat I. I. Abd-El-Moniem, Heba Hussein, Fatma M Darwish, Sara M. Elnomrosy, Naglaa Hagag, Essam M. Ibraheem, Mohamed Attya, Rawhya Emran, Hanan A. Fahmy, Ahmed R. Habashi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Sequence analysis Veterinary medicine viruses Papillomatosis phylogeny SF1-1100 SF600-1100 transmission electron microscopy medicine bovine papillomaviruses-2 Gene first detection Bovine papillomavirus General Veterinary biology Strain (chemistry) biology.organism_classification Molecular biology Animal culture GenBank immunohistochemistry histopathology Histopathology medicine.symptom Oncovirus Research Article |
| Zdroj: | Veterinary World Veterinary World, Vol 14, Iss 9, Pp 2296-2305 (2021) |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Background and Aim: Bovine papillomaviruses (BPV) are a heterogeneous group of oncoviruses, distributed globally, which produce major economic losses. In the current study, we compared the results of different diagnostic approaches and compared the strains identified in this study with previously characterized strains at local and international levels. Materials and Methods: Samples of skin warts were collected from five bovines with generalized papillomatosis from two Egyptian provinces, Menya and Ismailia, in 2020. Electron microscopy, molecular characterization, histopathological, and immunohistochemical examination were performed. Results: BPV was detected using electron microscopy in the collected samples. Using molecular characterization, BPV-2 was successfully identified for 1st time in Egypt. The strain has 99.6% identity with the BPV-2 reference strains obtained from GenBank. These results were supported by histopathology and immunohistochemistry examination. Partial nucleotide sequences of the L1 gene were submitted to GenBank with accession numbers MW289843 and MW289844. Conclusion: BPV-2 was reported for 1st time in the current study. The strain was identified grossly, microscopically, and pathologically and confirmed using molecular approaches. All results were consistent. The sequence analysis revealed that this strain has high sequence similarity to the reference Deltapapillomavirus-4, BPV-2 strains from Brazil and China. |
| Databáze: | OpenAIRE |
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