In ovo inoculation of duck embryos with different strains of Bacillus cereus to analyse their synergistic post‐hatch anti‐allergic potentialities

Autor:	Sana Mohammad Mahbubur Rahman, Mohammad Faysal Al Mazid, Salauddin Al Azad, KM Hossain, Pallob Barai, Mohammad Shamim Gazi
Rok vydání:	2020
Předmět:	Embryo Nonmammalian Antibiotic sensitivity Bacillus cereus Biology Injections law.invention Microbiology Probiotic law probiotic properties Anti-Allergic Agents Animals 16S rRNA gene sequencing Ovum Colony-forming unit in ovo inoculation antimicrobial activity General Veterinary Probiotics fungi Embryonated Original Articles Antimicrobial biology.organism_classification Ducks Cereus anti‐allergic potentialities bacteria Original Article Coagulase
Zdroj:	Veterinary Medicine and Science
ISSN:	2053-1095
DOI:	10.1002/vms3.279
Popis:	Background Bacillus cereus is a Gram‐positive, facultative anaerobic bacteria with few strains reported to be used as probiotics for animals and birds in recent times if the doses are formulated properly. Objectives To analyse the synergistic anti‐allergic potentiality of different Bacillus cereus strains on experimental in ovo and in vitro duck model, as probiotic immune stimulant. Materials and methods Different strains of Bacillus cereus from 29 isolates were identified through 16S rRNA gene sequencing from the milk samples of buffalo breeds of South Asia. The probiotic properties were tested in aspects of gram staining, catalase test, coagulase, test, bile salt tolerance, pH tolerance and phenol tolerance test. MIC50 and MIC90 levels were profiled using nine different antibiotics, and antimicrobial activity against eight different enteric pathogens was assessed. Finally, the test strains of Bacillus cereus (Colony Forming Unit [CFU] 30X1011) were combined‐infused at different concentrations in embryonated duck eggs to assess the post‐hatch anti‐allergic effects against histamine‐induced allergic reaction and their immunoglobulin E (IgE) level was tested. Results Molecular identification confirmed the test strains as B. cereus HKS 1–1, B. cereus LOCK 1,002 and B. cereus BF2, which were all motile, spore‐forming, catalase‐positive and rod‐shaped. All were 0.3% bile salt, 0.4% phenol and pH tolerant. Two‐way ANOVA test P values revealed that B. cereus BF2 was statistically significant (p Different Bacillus cereus strains were isolated from different buffalo milk samples to analyse their physiological, biochemical and molecular characterization. Their antibiotic sensitivity and antimicrobial activity were also tested to understand whether or not they can be used as therapeutic probiotics. Afterwards, formulation of the test strains at same CFU but at different concentrations was done to apply on the experimental farmed‐ducks to assess their efficacy in suppressing the level of allergy.
Databáze:	OpenAIRE
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