New Class of Drug Modalities: Directed Evolution of a De Novo Designed Helix–Loop–Helix Peptide to Bind VEGF for Tumor Growth Inhibition

Autor:	Masataka Michigami, Tharanga M. R. Ramanayake Mudiyanselage, Miho Suzuki, Hirotsugu Ishizako, Kunpei Notsu, Kikuya Sugiura, Ikuo Fujii
Rok vydání:	2022
Předmět:	Vascular Endothelial Growth Factor A Mice Peptide Library Neoplasms Human Umbilical Vein Endothelial Cells Animals Humans Molecular Medicine General Medicine Peptides Biochemistry
Zdroj:	ACS Chemical Biology. 17:647-653
ISSN:	1554-8937 1554-8929
DOI:	10.1021/acschembio.1c00940
Popis:	As a small affinity molecule to serve as an alternative to antibodies, we have developed a conformationally constrained peptide with a de novo designed helix-loop-helix (HLH) scaffold. To evaluate its potential for biomedical applications, we performed directed evolution of HLH peptides to obtain an inhibitor for vascular endothelial growth factor-A (VEGF). A phage-displayed library of HLH peptides was constructed and screened against VEGF, giving the peptide VS42 that inhibits the VEGF/VEGF receptor-2 interaction (IC
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60a58d4bea81688f71fbb748df821344 https://doi.org/10.1021/acschembio.1c00940 Zobrazit plný text záznamu