Establishment of a Bivector Genetic Transformation System in Recalcitrant Maize Inbred Lines
| Autor: | Rentao Song, Yajing Gu, Weiwei Qi, Xuan Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine business.industry Agriculture (General) Plant Science Biology maize bivector system 01 natural sciences Biotechnology S1-972 Crop 03 medical and health sciences Transformation (genetics) 030104 developmental biology Inbred strain Zheng58 genetic transformation Target gene business Agronomy and Crop Science Bivector transformation cycle 010606 plant biology & botany Food Science |
| Zdroj: | Agriculture Volume 11 Issue 7 Agriculture, Vol 11, Iss 663, p 663 (2021) |
| ISSN: | 2077-0472 |
| DOI: | 10.3390/agriculture11070663 |
| Popis: | Maize is an important grain crop with high nutritional value. An effective transformation system is crucial for the genetic improvement of maize traits, but many important maize inbred lines remained recalcitrant to transformation. In this study, we developed a bivector transformation system that worked well in two recalcitrant maize inbred lines. This system included an induction vector (ZmBBM-ZmWUS) and an indicator vector (GFP), using microprojectile bombardment technology combined with Agrobacterium-mediated transformation. We found that the Zheng58 and Mo17 recalcitrant inbred lines could be transformed with this system. The whole transformation cycle lasted only 52 days, 38 days less than the traditional transformation cycle. Additionally, it was possible to eliminate inference of the induction vector and obtained progenies with only the target gene. Our results suggested that the bivector system was an optimization of the current maize transformation methods and could potentially be used in genetic improvement of maize inbred lines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|