Identification of broadly protective human antibodies to Pseudomonas aeruginosa exopolysaccharide Psl by phenotypic screening
| Autor: | Ralph Minter, Ashley E. Keller, Vignesh Venkatraman, Michael P. McCarthy, Maria Margarita Camara, Sandrine Guillard, JoAnn Suzich, Lutz Jermutus, Peter Ravn, C. Kendall Stover, Qun Wang, Mladen Tomich, Paul Warrener, Jessica Bonnell, David Melnick, Jia Lin, Melissa Hamilton, Randall S. MacGill, Antonio DiGiandomenico |
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| Rok vydání: | 2012 |
| Předmět: |
Serotype
medicine.drug_class Phenotypic screening Immunology Antibiotics macromolecular substances Kaplan-Meier Estimate Biology Monoclonal antibody medicine.disease_cause Bacterial Adhesion Article Epitope Microbiology Mice Peptide Library Cell Line Tumor medicine Animals Humans Immunology and Allergy Pseudomonas Infections Serotyping Mice Inbred BALB C Mice Inbred C3H Microbial Viability Pseudomonas aeruginosa Polysaccharides Bacterial Biofilm Antibodies Monoclonal Pneumonia Virology Mutation biology.protein Antibody Single-Chain Antibodies |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.20120033 |
| Popis: | A human antibody facilitates opsonophagocytic killing, inhibits attachment of Pseudomonas aeruginosa, and exerts protective effects in several animal models of P. aeruginosa infection. Pseudomonas aeruginosa is a leading cause of hospital-associated infections in the seriously ill, and the primary agent of chronic lung infections in cystic fibrosis patients. A major obstacle to effective control of P. aeruginosa infections is its intrinsic resistance to most antibiotic classes, which results from chromosomally encoded drug-efflux systems and multiple acquired resistance mechanisms selected by years of aggressive antibiotic therapy. These factors demand new strategies and drugs to prevent and treat P. aeruginosa infections. Herein, we describe a monoclonal antibody (mAb) selection strategy on whole P. aeruginosa cells using single-chain variable fragment phage libraries derived from healthy individuals and patients convalescing from P. aeruginosa infections. This approach enabled identification of mAbs that bind three distinct epitopes on the product of the Psl. This exopolysaccharide is important for P. aeruginosa attachment to mammalian cells, and for the formation and maintenance of biofilms produced by nonmucoid and mucoid P. aeruginosa isolates. Functional screens revealed that mAbs to one epitope exhibit superior activity in opsonophagocytic killing and cell attachment assays, and confer significant protection in multiple animal models. Our results indicate that Psl is an accessible serotype-independent surface feature and promising novel protective antigen for preventing P. aeruginosa infections. Furthermore, our mAb discovery strategy holds promise for application to other bacterial pathogens. |
| Databáze: | OpenAIRE |
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