Insulin-like growth factor II (IGF-II) mRNA expression during skeletal muscle development of doubled-muscled and normal bovine foetuses
| Autor: | Anne Listrat, Brigitte Picard, Lucette Belair, Hélène Jammes, Nathalie Boulle, Jean Djiane, Yves Geay |
|---|---|
| Přispěvatelé: | Revues Inra, Import |
| Předmět: |
medicine.medical_specialty
Gene Expression Gestational Age Biology Andrology 03 medical and health sciences Insulin-Like Growth Factor II Internal medicine Gene expression [SDV.BDD] Life Sciences [q-bio]/Development Biology medicine Animals Humans Northern blot RNA Messenger Autocrine signalling Muscle Skeletal [SDV.BDD]Life Sciences [q-bio]/Development Biology ComputingMilieux_MISCELLANEOUS [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology 030304 developmental biology 0303 health sciences Messenger RNA Hyperplasia Muscle cell differentiation 0402 animal and dairy science Skeletal muscle [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology 04 agricultural and veterinary sciences Blotting Northern 040201 dairy & animal science Actins Blot [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition medicine.anatomical_structure Endocrinology Insulin-like growth factor 2 biology.protein Cattle [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | HAL Reproduction Nutrition Development Reproduction Nutrition Development, EDP Sciences, 1999, 39 (1), pp.113-124 |
| ISSN: | 0926-5287 1297-9708 |
| Popis: | We investigated the IGF-II gene expression in developing Semitendinosus muscle in foetal normal and double-muscled cattle. Samples from normal and double-muscled foetuses ranging from 90 to 210 d post-conception were collected and total RNA extracted. Northern blot analysis was performed using the human IGF-II cDNA probe. Five IGF-II transcripts, 5.1, 4.4, 3.7, 2.6 and 1.7 kb, were detected in muscle samples. Throughout gestation, all transcripts, except for the 5.1 kb one, decreased similarly in both genetic types. In double-muscled foetuses, the amount of the 5.1 kb transcript was higher than those of the other transcripts and its expression remained stable throughout the gestational stages analysed. These results indicated that the regulation of IGF-II gene transcription was distinct in both genetic types. The IGF-II foetal plasma concentrations increased throughout gestation. In bovine foetuses, the first muscle cell differentiation was concomitant with a high autocrine IGF-II mRNA expression and low plasma IGF-II levels in both genetic types. The second step of muscle cell differentiation was associated with high IGF-II plasma concentrations and the autocrine expression of IGF-II was reduced. |
| Databáze: | OpenAIRE |
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