Antinociceptive effect of triterpene acetyl aleuritolic acid isolated from Croton zehntneri in adult zebrafish (Danio rerio)
| Autor: | Emmanuel Silva Marinho, Emanuelle Machado Marinho, Paulo Nogueira Bandeira, Hélcio Silva dos Santos, Francisco Ernani Alves Magalhães, Jane Eire Silva Alencar de Menezes, Francisca Crislândia Oliveira Silva, Maria Kueirislene Amâncio Ferreira, Alexandre Magno Rodrigues Teixeira, Márcia Machado Marinho, Antonio Wlisses da Silva, Antonia Jaqueline Nobre Bezerra |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Models Molecular Nociception Analgesic Biophysics TRPV1 Palmitic Acids Pharmacology Biochemistry Cornea 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animals Molecular Biology Zebrafish Analgesics Cell Biology Acute toxicity Triterpenes Hypertonic saline 030104 developmental biology chemistry Capsaicin 030220 oncology & carcinogenesis Toxicity Croton Capsazepine |
| Zdroj: | Biochemical and biophysical research communications. 534 |
| ISSN: | 1090-2104 |
| Popis: | Croton zehntneri is a plant known as canelinha de cunha, prevalent in the northeast region of Brazil. Many constituents of the vegetable have already been studied, and their pharmacological properties have been proven, but this is the first study to analyze the antinociceptive effect in adult zebrafish (ZFa) of the triterpene acetyl aleuritolic acid (AAA) isolated from the stem bark. The animals (ZFa; n = 6/group) were treated intraperitoneally (ip; 20 μL) with AAA (0.1 or 0.3 or 1.0 mg/mL) or vehicle (0.9% saline; 20 μL), and submitted to the locomotor activity test, as well as 96 h acute toxicity. Other groups (n = 6/each) received the same treatments and underwent acute nociception tests (formalin, cinnamaldehyde, glutamate, acid saline, capsaicin, and hypertonic saline). Possible neuromodulation mechanisms were evaluated. AAA (0.1 or 0.3 or 1.0 mg/mL) reduced the nociceptive behavior induced by acid saline and capsaicin, as well as inhibited corneal nociception induced by hypertonic saline, both without altering the animals’ locomotor system and without toxicity. These analgesic effects of AAA were significantly (p > 0.05) similar to those of morphine, used as a positive control. The antinociceptive effect of AAA was inhibited by methylene blue, ketamine, camphor, ruthenium red, amiloride, and mefenamic acid. The antinociceptive effect of AAA on the cornea of animals was inhibited by capsazepine. Therefore, AAA showed pharmacological potential for the treatment of acute pain, and this effect is modulated by cGMP, NMDA receptors, transient receptor potential channels (TRPs), ASICs and has pharmacological potential for the treatment of corneal pain modulated by the TRPV1 channel. |
| Databáze: | OpenAIRE |
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