Evaluation of fungal chitosan as a biocontrol and antibacterial agent using fluorescence-labeling
| Autor: | Ahmad I. Al-Turki, Ahmed A. Tayel, Shaaban H. Moussa |
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| Rok vydání: | 2012 |
| Předmět: |
Lysis
macromolecular substances Bacterial growth Polysaccharide Biochemistry Bacterial cell structure Fluorescence Microbiology Chitosan chemistry.chemical_compound Structural Biology Escherichia coli Molecular Biology Antibacterial agent chemistry.chemical_classification biology Staining and Labeling technology industry and agriculture General Medicine equipment and supplies biology.organism_classification Antimicrobial Anti-Bacterial Agents carbohydrates (lipids) chemistry Biological Control Agents Aspergillus niger Bacteria Fluorescein-5-isothiocyanate |
| Zdroj: | International journal of biological macromolecules. 54 |
| ISSN: | 1879-0003 |
| Popis: | Chitosan is a precious biological polysaccharide that could be applied in several fields. Fluorescein isothiocyanate-labeled chitosan (FITC-CTS) was synthesized as a macromolecular fluorophore added to fungal chitosan from Aspergillus niger, to investigate the interaction mechanism and antibacterial performance of (FITC-CTS) against Escherichia coli and Micrococcus leteus. Fluorescence imaging was used to demonstrate chitosan effect on the bacterial cells. Fluorescence density of treated bacteria with FITC-CTS was correlated with viable cell number. Electron micrographs of treated E. coli with fungal chitosan revealed that chitosan principally interact with bacterial cell wall, causing cell wall lyses with exposure time prolongation. Fungal chitosan could be proposed for bacterial growth control as a powerful, natural and safe alternative to synthetic and chemical bactericides. Fluorescence labeling proved to be an efficient tool for determining the antimicrobial activity of chitosan. |
| Databáze: | OpenAIRE |
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