Immunological properties of the cell surface haemagglutinins (sHAs) ofHelicobacter pyloristrain NCTC 11637
| Autor: | Torkel Wadström, Janaki Lelwala-Guruge, Åsa Ljungh, Arnold S. Kreger |
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| Rok vydání: | 1995 |
| Předmět: |
Microbiology (medical)
Antigenicity Immunology Enzyme-Linked Immunosorbent Assay Cross Reactions Biology Microbiology stomatognathic system Neutralization Tests Animals Humans Immunology and Allergy Immunoelectrophoresis chemistry.chemical_classification Antiserum Helicobacter pylori Strain (chemistry) General Medicine Hemagglutinin Precipitin Antibodies Bacterial Molecular biology Titer Hemagglutinins Infectious Diseases Biochemistry chemistry Immunoglobulin G Antigens Surface biology.protein Rabbits Antibody Glycoprotein |
| Zdroj: | FEMS Immunology & Medical Microbiology. 11:73-77 |
| ISSN: | 1574-695X 0928-8244 |
| DOI: | 10.1111/j.1574-695x.1995.tb00081.x |
| Popis: | Rabbits were immunised with stage 1 and stage 2 soluble haemagglutinins (sHA) of Helicobacter pylori strain NCTC 11637 and with rabbit erythrocytes coated with stage 1 sHA. After adsorption of stage 1 sHA on erythrocytes, SDS-PAGE analysis showed that 4 major protein bands were removed from the preparation. The anti-sHA coated erythrocyte serum had the highest HA inhibition titre of 16. Crossed immunoelectrophoresis of the stage 1 sHA, against stage 1 and 2 antisera showed multiple precipitin arcs; however, the anti-sHA coated erythrocyte serum produced only two arcs. One arc produced by the anti-stage 2 serum was absent with the anti-stage 1 serum. This arc could have been produced against a 20 kDa polypeptide which was absent in the stage 1 sHA. The other arc was stronger when compared with that produced by anti-stage 1 serum. These two arcs corresponded to the two arcs produced by the anti-sHA coated erythrocyte serum, which had the highest inhibition titre. The two arcs were markedly reduced in crossed immunoelectrophoresis with an adsorbed stage 1 sHA preparation, which indicates that these arcs were produced against the sHAs. |
| Databáze: | OpenAIRE |
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