Impact of a novel protein meal on the gastrointestinal microbiota and host transcriptome of larval zebrafish Danio rerio
| Autor: | Gianina M. Bacanu, Jeroen Kals, Arjan P. Palstra, Maria Forlenza, Detmer Sipkema, Hauke Smidt, E. Rurangwa, Menno ter Veld |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
intestinal microbiota
16S rRNA-based microbial composition Firmicutes Physiology solea-senegalensis Danio zebrafish nutrition mRNA sequencing Celbiologie en Immunologie Aquaculture Gut flora Microbiology lcsh:Physiology Transcriptome iron Aquaculture and Fisheries Microbiologie large gene lists Physiology (medical) expression metal uptake iron metabolism gastrointestinal tract transcriptome Zebrafish Original Research Genetics fish biology gut microbiota lcsh:QP1-981 Aquacultuur en Visserij biology.organism_classification digestive physiology Aquacultuur MRNA Sequencing pyrosequencing Cell Biology and Immunology WIAS Pyrosequencing Departement Dierwetenschappen Proteobacteria sp-nov Department of Animal Sciences |
| Zdroj: | Frontiers in Physiology, Vol 6 (2015) Frontiers in Physiology 6 (2015) Frontiers in Physiology Frontiers in Physiology, 6 |
| ISSN: | 1664-042X |
| DOI: | 10.3389/fphys.2015.00133/full |
| Popis: | Larval zebrafish was subjected to a methodological exploration of the gastrointestinal microbiota and transcriptome. Assessed was the impact of two dietary inclusion levels of a novel protein meal (NPM) of animal origin (ragworm Nereis virens) on the gastrointestinal tract (GIT). Microbial development was assessed over the first 21 days post egg fertilization (dpf) through 16S rRNA gene-based microbial composition profiling by pyrosequencing. Differentially expressed genes in the GIT were demonstrated at 21 dpf by whole transcriptome sequencing (mRNAseq). Larval zebrafish showed rapid temporal changes in microbial colonization but domination occurred by one to three bacterial species generally belonging to Proteobacteria and Firmicutes. The high iron content of NPM may have led to an increased relative abundance of bacteria that were related to potential pathogens and bacteria with an increased iron metabolism. Functional classification of the 328 differentially expressed genes indicated that the GIT of larvae fed at higher NPM level was more active in transmembrane ion transport and protein synthesis. mRNAseq analysis did not reveal a major activation of genes involved in the immune response or indicating differences in iron uptake and homeostasis in zebrafish fed at the high inclusion level of NPM. |
| Databáze: | OpenAIRE |
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