Periostin (an osteoblast-specific factor) is expressed within the embryonic mouse heart during valve formation
| Autor: | Roger R. Markwald, Rhonda Rogers, Michal Machnicki, Simon J. Conway, Agnieszka Kruzynska-Frejtag |
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| Rok vydání: | 2001 |
| Předmět: |
Embryology
medicine.medical_specialty Heart morphogenesis DNA Complementary Time Factors Biology Periostin Bone morphogenetic protein Bone morphogenetic protein 2 Mice Internal medicine medicine Animals RNA Messenger In Situ Hybridization Embryonic heart Heart development Reverse Transcriptase Polymerase Chain Reaction Myocardium Heart Osteoblast Heart Valves Cell biology Endocrinology medicine.anatomical_structure Cell Adhesion Molecules Developmental Biology Transforming growth factor |
| Zdroj: | Mechanisms of Development. 103:183-188 |
| ISSN: | 0925-4773 |
| Popis: | Periostin was originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. Likewise the endocardial cushions that form within embryonic heart tube (embryonic day (E)10-13) are formed by the recruitment, attachment and spreading of endocardial cells into the overlying extracellular matrix, in response to secreted growth factors of the TGFbeta and BMP families. In order to determine whether periostin is similarly involved in heart morphogenesis, in situ hybridization and reverse transcription-polymerase chain reaction were used to detect periostin mRNA expression in the developing mouse heart. We show for the first time that periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart. |
| Databáze: | OpenAIRE |
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