Diamine oxidase is involved in H2O2 production in the chalazal cells during barley grain filling
| Autor: | Carol M. Duffus, Bavita Asthir, Rachel C. Smith, William Spoor |
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| Rok vydání: | 2002 |
| Předmět: |
Spermidine
Physiology Spermine Plant Science Biology chemistry.chemical_compound Polyamines Putrescine Peroxidase Oxidoreductases Acting on CH-NH Group Donors Potassium Iodide Amine oxidase (copper-containing) Hordeum Starch Hydrogen Peroxide chemistry Biochemistry Seeds Amine Oxidase (Copper-Containing) Hordeum vulgare Isoelectric Focusing Diamine oxidase Polyamine Polyamine oxidase |
| Zdroj: | Journal of Experimental Botany. 53:677-682 |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jexbot/53.369.677 |
| Popis: | The localization and activities of diamine oxidase (DAO, EC 1.4.3.6) and polyamine oxidase (PAO, EC 1.4.3.4) together with polyamine levels have been investigated in developing grains of barley (Hordeum vulgare L.). DAO (pH 7.5) is present mainly in vascular tissue and its neighbouring cells, namely chalazal cells and nucellar projection, while PAO (pH 6.0) is mainly localized in the chlorenchymatous cells of the crease and at the base of the vascular tissue. Activities of both these enzymes appear to be independently-regulated, as DAO activity increased steadily throughout grain development while PAO activity was higher during the early stages of grain filling, declined thereafter and again increased towards maturity. The maximum activities of DAO coincided with the maximum content of putrescine while the levels of PAO did not seem to be directly correlated with spermidine or spermine contents. Isoelectric focusing (IEF) of DAO and PAO activities revealed the presence of bands at 30 and 45 DPA. The possible involvement of DAO and PAO in the supply of H(2)O(2) to peroxidase-catalysed reactions in the chalazal cells during grain filling is discussed. |
| Databáze: | OpenAIRE |
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