Inhibitory Effects of Diclofenac on Steroid Glucuronidation In Vivo Do Not Affect Hair-Based Doping Tests for Stanozolol

Autor: James Barker, Andrea Petróczi, Declan P. Naughton, Gergely Zachar, Iltaf Shah, Nawed Deshmukh, Andrea Székely
Rok vydání: 2017
Předmět:
medicine.medical_treatment
Glucuronidation
Pharmaceutical Science
Urine
Pharmacology
Analytical Chemistry
0302 clinical medicine
Anabolic Agents
anti-inflammatory drug
Drug Discovery
Stanozolol
Doping in Sports
medicine.diagnostic_test
Chemistry
steroid
inhibition
metabolism
Substance Abuse Detection
Chemistry (miscellaneous)
Molecular Medicine
Steroids
medicine.drug
pharmacy
medicine.medical_specialty
Diclofenac
Urinalysis
endocrinology_metabolomics
chemistry
Gas Chromatography-Mass Spectrometry
Article
Steroid
Excretion
lcsh:QD241-441
03 medical and health sciences
Glucuronides
lcsh:Organic chemistry
In vivo
Internal medicine
medicine
Animals
Humans
Physical and Theoretical Chemistry
Organic Chemistry
030229 sport sciences
Rats
stomatognathic diseases
Endocrinology
030217 neurology & neurosurgery
biological
Hair
Zdroj: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Molecules
Molecules, Vol 22, Iss 6, p 976 (2017)
Molecules; Volume 22; Issue 6; Pages: 976
ISSN: 1420-3049
Popis: In vitro studies show that diclofenac inhibits enzymatic steroid glucuronidation. This study was designed to investigate the influence of diclofenac on the excretion of stanozolol and 3'-hydroxystanozolol via analyses in hair, blood and urine in vivo in a rat study. Brown Norway rats were administered with stanozolol (weeks 1-3) and diclofenac (weeks 1-6). Weekly assessment of steroid levels in hair was complemented with spot urine and serum tests. Levels of both stanozolol and 3'-hydroxystanozolol steadily increased in hair during stanozolol treatment and decreased post-treatment, but remained readily detectable for 6 weeks. In contrast, compared to control rats, diclofenac significantly reduced urinary excretion of 3’-hydroxystanozolol which was undetectable in most samples. This is the first report of diclofenac altering steroid metabolism in vivo, detrimentally affecting detection in urine, but not in hair which holds considerable advantages over urinalysis for anti-doping tests.
Databáze: OpenAIRE
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