Versatile Functionalization of P25 Conjugated ND Nanocomposites for UV-Mediated Free Radical Scavenging and Facilitates Anti-Inflammation Potential in Human Cells
| Autor: | Er Chieh Cho, Yi Lun Chen, Guang Yu Lee, Ming Yen Sung, Jen Hsien Huang, Kuen Chan Lee |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Antioxidant Cell Survival Surface Properties Ultraviolet Rays medicine.medical_treatment Radical Anti-Inflammatory Agents Conjugated system medicine.disease_cause Theranostic Nanomedicine Nanocomposites Nanodiamonds medicine Animals Humans General Materials Science Viability assay Zebrafish Cell Proliferation chemistry.chemical_classification Titanium Reactive oxygen species Esterification Cell growth Free Radical Scavengers Amides Carbodiimides Oxidative Stress Cross-Linking Reagents HEK293 Cells chemistry Models Animal Biophysics Surface modification Reactive Oxygen Species Oxidative stress |
| Zdroj: | ACS applied materialsinterfaces. 13(33) |
| ISSN: | 1944-8252 |
| Popis: | In this work, we demonstrated that building different linking groups between nanodiamond (ND) and TiO2 (P25) could provide more effective protection under oxidative stress and ultraviolet (UV) light irradiation compared with the use of TiO2 alone. The establishment of ester (-C-O-O-R), amide (-CONH-), and epoxide-amine adduct (-NHCCO-) groups between ND-TiO2 composites was found to be critical in the generation of reactive oxygen species (ROS) by controlling their charge transfer behaviors. We hypothesized that linking groups between the composites dictate the performance of ROS generation from nano-TiO2 under UV-light irradiation due to the differences in linking groups. The results showed that hydroxyl radicals were attenuated by the incorporation of ND. An MTT cell proliferation assay was performed in human cells under the treatment of ND-TiO2 composites to investigate the impacts of composites on cell viability. The results from the luciferase reporter assay suggested they have anti-inflammatory activity and can reduce cellular DNA damage under ROS stimulation. A zebrafish model was also applied with the ND-TiO2 composite treatment to demonstrate the safety aspects of the composites in vivo and their biomedical application potential. Studies exploring ROS generation behaviors in different linking groups suggested that interactive functionalization between nanoparticles might be an ideal antioxidant and anti-inflammatory strategy. |
| Databáze: | OpenAIRE |
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