The Expression Profile of mRNA and tRNA Genes in Splenocytes and Neutrophils after In Vivo Delivery of Antitumor Short Hairpin RNA of Indoleamine 2,3- Dioxygenase
| Autor: | I-Jeng Yeh, Kuan-Ting Liu, Meng-Chi Yen, Ming-Shyan Huang, Ya-Ling Hsu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Lung Neoplasms
short-hairpin RNA (shRNA) Neutrophils non-coding RNA Antineoplastic Agents Biology animal tumor model Article Catalysis lcsh:Chemistry Inorganic Chemistry Small hairpin RNA Mice RNA Transfer Cell Line Tumor indoleamine 2 3-dioxygenase1 (IDO1) Biomarkers Tumor Splenocyte Animals Indoleamine-Pyrrole 2 3 -Dioxygenase RNA Messenger RNA Small Interfering Physical and Theoretical Chemistry Indoleamine 2 3-dioxygenase lcsh:QH301-705.5 Molecular Biology Gene Spectroscopy Messenger RNA Organic Chemistry neutrophil RNA RNA sequencing General Medicine Non-coding RNA Molecular biology Computer Science Applications Mice Inbred C57BL transfer RNA (tRNA) Gene Ontology lcsh:Biology (General) lcsh:QD1-999 Gene Expression Regulation Transfer RNA biomarker Female Spleen |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 6703, p 6703 (2020) Volume 21 Issue 18 |
| ISSN: | 1422-0067 |
| Popis: | RNA-based therapeutics are considered as novel treatments for human diseases. Our previous study demonstrated that treatment with short-hairpin RNA against Ido1 (IDO shRNA) suppresses tumor growth, detects Th1-bias immune responses, and elevates expression of tryptophan transfer RNA (tRNATrp) in total splenocytes. In addition, depletion of Ly6g+ neutrophils attenuates the effect of IDO shRNA. The aim of this study was to investigate the regulatory network and the expression profile of tRNAs and other non-coding RNAs in IDO shRNA-treated spleens. The total splenocytes and magnetic bead-enriched splenic neutrophils were collected from the lung tumor bearing mice, which were treated with IDO shRNA or scramble IDO shRNA, and the collected cells were subsequently subjected to RNA sequencing. The gene ontology analysis revealed the different enrichment pathways in total splenocytes and splenic neutrophils. Furthermore, the expression of tRNA genes was identified and validated. Six isoacceptors of tRNA, with different expression patterns between total splenocytes and splenic neutrophils, were observed. In summary, our findings not only revealed novel biological processes in IDO shRNA-treated total splenocytes and splenic neutrophils, but the identified tRNAs and other non-coding RNAs may contribute to developing a novel biomarker gene set for evaluating the clinical efficiency of RNA-based cancer immunotherapies. |
| Databáze: | OpenAIRE |
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