Portable electrochemical immunosensor for detection of Mycobacterium tuberculosis secreted protein CFP10-ESAT6 in clinical sputum samples
| Autor: | Nor Azah Yusof, Shahrul Ainliah Alang Ahmad, Umi Zulaikha Mohd Azmi, Nur Khairunnisa Sahar, Siti Suraiya, Devandran Krishnan, Fatin Nabilah Mohd Faudzi, Poh Shing Ong, Jaafar Abdullah, Nurul Hanun Ahmad Raston |
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| Rok vydání: | 2020 |
| Předmět: |
Tuberculosis
Recombinant Fusion Proteins 02 engineering and technology Electrochemistry 01 natural sciences Sensitivity and Specificity Analytical Chemistry Mycobacterium tuberculosis Antigen Bacterial Proteins Limit of Detection medicine Humans Magnetite Nanoparticles Detection limit Immunoassay Chromatography biology Chemistry 010401 analytical chemistry Sputum Reproducibility of Results Electrochemical Techniques 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease 0104 chemical sciences Linear range Differential pulse voltammetry Gold medicine.symptom 0210 nano-technology |
| Zdroj: | Mikrochimica acta. 188(1) |
| ISSN: | 1436-5073 |
| Popis: | An early detection of Mycobacterium tuberculosis is very important to reduce the number of fatal cases and allow for fast recovery. However, the interpretation of the result from smear microscopy requires skilled personnel due to the propensity of the method to produce false-negative results. In this work, a portable, rapid, and simple sandwich-type immunosensor reader has been developed that is able to detect the presence of M. tuberculosis in sputum samples. By using sandwich-type immunosensor, an anti-CFP10-ESAT6 antibody was immobilized onto the graphene/polyaniline (GP/PANI)-modified gold screen-printed electrode. After incubation with the target CFP10-ESAT6 antigen, the iron/gold magnetic nanoparticles (Fe3O4/Au MNPs) conjugated with anti-CFP10-ESAT6 antibody were used to complete the sandwich format. Differential pulse voltammetry (DPV) technique was used to detect the CFP10-ESAT6 antigen at the potential range of 0.0–1.0 V. The detection time is less than 2 h. Under optimal condition, CFP10-ESAT6 antigen was detected in a linear range from 10 to 500 ng mL−1 with a limit of detection at 1.5 ng mL−1. The method developed from this process was then integrated into a portable reader. The performance of the sensor was investigated and compared with the standard methods (culture and smear microscopy). It provides a good correlation (100% sensitivity and 91.7% specificity) with both methods of detection for M. tuberculosis in sputum samples henceforth, demonstrating the potential of the device as a more practical screening tool. Graphical abstract |
| Databáze: | OpenAIRE |
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