Use of Quantitative 16S Ribosomal DNA Detection for Diagnosis of Central Vascular Catheter-Associated Bacterial Infection
| Autor: | Simon Warwick, M. Small, Mark Wilks, J. Powell-Tuck, J. Sharp, Michael Millar, Enid M. Hennessy |
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| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Catheterization Central Venous Vascular catheter Population DNA Ribosomal Polymerase Chain Reaction law.invention Microbiology law RNA Ribosomal 16S Humans education Ribosomal DNA Polymerase chain reaction DNA Primers education.field_of_study biology Bacteriology Bacterial Infections equipment and supplies biology.organism_classification Virology Real-time polymerase chain reaction Intravenous nutrition Bacteria Bacterial dna |
| Zdroj: | Journal of Clinical Microbiology. 42:1402-1408 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.42.4.1402-1408.2004 |
| Popis: | Many central vascular catheters (CVCs) are removed unnecessarily because current diagnostic methods for CVC-associated infection are unreliable. A quantitative PCR assay using primers and probe targeted to bacterial 16S ribosomal DNA was used to measure the levels of bacterial DNA in blood samples drawn through the CVC in a population of patients receiving intravenous nutrition. Bacterial DNA concentrations were raised in 16 of 16 blood samples taken during episodes of probable bacterial CVC-associated infection. Bacterial DNA concentrations were raised in 4 of 29 episodes in which bacterial CVC-associated infection was unlikely. The use of this technique has the potential to substantially reduce the unnecessary removal of CVCs. |
| Databáze: | OpenAIRE |
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