Effects of pentosan polysulfate and polysulfated glycosaminoglycan on chondrogenesis of canine bone marrow-derived mesenchymal stem cells in alginate and micromass culture
| Autor: | Masahiro Okumura, Sangho Kim, Jing Fang, Eugene C Bwalya, Kenji Hosoya, H. M. Suranji Wijekoon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
polysulfated glycosaminoglycan 040301 veterinary sciences Alginates education Type II collagen Real-Time Polymerase Chain Reaction 0403 veterinary science Andrology 03 medical and health sciences Polysulfated glycosaminoglycan pentosan polysulfate Chondrocytes Dogs Glucuronic Acid medicine Animals Dog Diseases Aggrecan Cells Cultured Glycosaminoglycans Pentosan Sulfuric Polyester General Veterinary Full Paper Chemistry Cartilage Hexuronic Acids Mesenchymal stem cell Mesenchymal Stem Cells 04 agricultural and veterinary sciences Pentosan polysulfate Chondrogenesis osteoarthritis 030104 developmental biology medicine.anatomical_structure cartilage tissue regeneration canine bone marrow-derived mesenchymal stem cells Surgery Type I collagen medicine.drug |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Mesenchymal stem cells (MSC) are a potential alternative source of differentiated chondrocytes for cartilage tissue regeneration and repair of osteoarthritic (OA) joints. We investigated the effects of pentosan polysulfate (PPS) and polysulfated glycosaminoglycan (PSGAG) on chondrogenesis of canine bone marrow-derived mesenchymal stem cells (cBMSC) in alginate and micromass cultures (MMC). Chondrogenic differentiation medium (CDM) was supplemented with PPS or PSGAG at concentrations of 0 (positive control; PC), 1, 3 and 5 µg/ml. 10% DMEM was used as negative control. Chondrocyte phenotype was analyzed by quantitative real-time PCR (qPCR) for alginate cultures and Alcian blue staining for proteoglycan (PG) synthesis for MMC. In alginate culture, PPS and PSGAG showed no significant effect on type II collagen, aggrecan and HIF-2α mRNA expression. PPS had no significant effect on type I collagen whereas PSGAG significantly upregulated (P |
| Databáze: | OpenAIRE |
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