Ectopic expression of mutated type 2C protein phosphatase OsABI-LIKE2 decreases abscisic acid sensitivity in Arabidopsis and rice
| Autor: | Etsuo Shimosaka, Mihoko Oohashi, Akira Endo, Yutaka Sato, Ayano Meguro-Maoka, Chika Egawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phosphatase Arabidopsis lcsh:Medicine Genetically modified crops 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Two-Hybrid System Techniques Aspartic acid lcsh:Science Abscisic acid Plant Proteins chemistry.chemical_classification Multidisciplinary biology fungi lcsh:R food and beverages Oryza biology.organism_classification Genetically modified rice Amino acid 030104 developmental biology chemistry Biochemistry Mutation Ectopic expression lcsh:Q Abscisic Acid 010606 plant biology & botany |
| Zdroj: | SC30201903070007 NARO成果DBa OA 表示-非営利-改変禁止(CC-BY-NC-ND) Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Abscisic acid (ABA) is a phytohormone that is necessary for stress adaptation. Recent studies have reported that attenuated levels of ABA improved grain yield and seedling growth under low temperature in cereals. To improve plant growth under low temperature, we attempted to generate ABA-insensitive transgenic rice by expressing a clade A type 2C protein phosphatase (OsPP2C), OsABIL2, with or without the mutation equivalent to the Arabidopsis abi1-1 mutation. A yeast two-hybrid assay revealed that the interaction between OsABIL2 and a putative rice ABA receptor, OsPYL1, was ABA-dependent, and the interaction was lost with amino acid substitution from glycine to aspartic acid at the 183rd amino acid of the OsABIL2 protein, corresponding to abi1-1 mutation. The constitutive expression of OsABIL2 or OsABIL2G183D in Arabidopsis or rice decreased ABA sensitivity to differing degrees. Moreover, the transgenic rice expressing OsABIL2G183D exhibited improved seedling growth under low temperature, although the transgenic lines showed unfavorable traits, such as viviparous germination and elongated internodes. These results indicated that the introduction of abi1-1 type dominant mutation was also effective in OsABIL2 at decreasing ABA sensitivity in plants, and the attenuation of ABA sensitivity could be an alternative parameter to improve rice performance under low temperatures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|