Inhibition of Src reduces gemcitabine-induced cytotoxicity in human pancreatic cancer cell lines
| Autor: | Naoto Ichihara, Tomohiko Taminato, Akira Kitanaka, Terukazu Tanaka, Yoshitsugu Kubota |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Serum
Cancer Research Antimetabolites Antineoplastic medicine.drug_class Cell Survival Apoptosis Transfection Deoxycytidine Tyrosine-kinase inhibitor Pancreatic cancer Cell Line Tumor Antineoplastic Combined Chemotherapy Protocols medicine Humans Cytotoxicity Protein Kinase Inhibitors Cell Proliferation Dose-Response Relationship Drug Cell growth Chemistry medicine.disease Gemcitabine Culture Media Pancreatic Neoplasms Pyrimidines src-Family Kinases Oncology Cell culture Mutation Cancer research Proto-oncogene tyrosine-protein kinase Src Signal Transduction |
| Zdroj: | Cancer letters. 260(1-2) |
| ISSN: | 0304-3835 |
| Popis: | In the present study, we examined the role of Src in gemcitabine-induced cell growth suppression in human pancreatic cancer cell lines. In two human pancreatic cancer cell lines, PK-9 and MIA PaCa-2, we found that a selective Src protein tyrosine kinase inhibitor, PP2, inhibited gemcitabine-induced cell growth suppression. When dominant negative src cDNA was constitutively expressed in PK-9 cells (PK-9-Src-DN), the degree of gemcitabine-induced cell growth suppression was decreased compared with that of mock-transfected PK-9 cells. The mechanism of the inhibitory effect of gemcitabine-induced cytotoxicity was found to be the suppression of apoptosis, which was downregulated in PK-9-Src-DN cells. These results indicate that Src mediates signals that culminate in suppressing cell growth and survival in the presence of gemcitabine, at least in particular cell lines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect