Comparação entre duas técnicas de análise de fibras elásticas pulmonares em coelhos prematuros

Autor: Mariana Mendes Silva, Renata Suman Mascaretti, Celso Moura Rebello, Fernanda Polastri Thereza, Bruno Garcia de Aquino, Roberta Munhoz Manzano, Rodrigo Leonel dos Santos
Jazyk: portugalština
Rok vydání: 2012
Předmět:
Zdroj: ConScientiae Saúde, Vol 11, Iss 2, Pp 217-224 (2012)
ISSN: 1983-9324
1677-1028
Popis: Introduction: Bronchopulmonary dysplasia is characterized by a blockage in lung development. Objective: To compare the efficiency of two techniques of analysis of elastic fibers in lungs of premature rabbits. Methods: Preterm rabbits born on the 28th and 29th days of gestation were subjected to ambient air or oxygen for 11 days, randomized into four groups, Ar 28 (n=17), O2 28 (n= 7), Ar 29 (n=8), O2 29 (n=8). The lungs were removed and stained for analysis of elastic fibers. The Nikkon E600 microscope and the image analysis software (Image- Pro Plus 4.5r) were used for elastic fiber analysis. To compare the analysis between microscope and program was used paired "t" test. Results: There were significant differences between the proportion of elastic fibers performed on the microscope and at the program (p
Databáze: OpenAIRE