Comparação entre duas técnicas de análise de fibras elásticas pulmonares em coelhos prematuros
Autor: | Mariana Mendes Silva, Renata Suman Mascaretti, Celso Moura Rebello, Fernanda Polastri Thereza, Bruno Garcia de Aquino, Roberta Munhoz Manzano, Rodrigo Leonel dos Santos |
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Jazyk: | portugalština |
Rok vydání: | 2012 |
Předmět: |
Microscope
lcsh:Diseases of the musculoskeletal system business.industry Chemistry General Medicine medicine.disease Ambient air law.invention medicine.anatomical_structure Bronchopulmonary dysplasia law medicine lcsh:RC925-935 lcsh:Sports medicine Nuclear medicine business lcsh:RC1200-1245 Elastic fiber |
Zdroj: | ConScientiae Saúde, Vol 11, Iss 2, Pp 217-224 (2012) |
ISSN: | 1983-9324 1677-1028 |
Popis: | Introduction: Bronchopulmonary dysplasia is characterized by a blockage in lung development. Objective: To compare the efficiency of two techniques of analysis of elastic fibers in lungs of premature rabbits. Methods: Preterm rabbits born on the 28th and 29th days of gestation were subjected to ambient air or oxygen for 11 days, randomized into four groups, Ar 28 (n=17), O2 28 (n= 7), Ar 29 (n=8), O2 29 (n=8). The lungs were removed and stained for analysis of elastic fibers. The Nikkon E600 microscope and the image analysis software (Image- Pro Plus 4.5r) were used for elastic fiber analysis. To compare the analysis between microscope and program was used paired "t" test. Results: There were significant differences between the proportion of elastic fibers performed on the microscope and at the program (p |
Databáze: | OpenAIRE |
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