Investigating the two regimes of fibrin clot lysis: an experimental and computational approach
| Autor: | David Perez-Morga, Franck Raynaud, Alexandre Rousseau, Bastien Chopard, Karim Zouaoui Boudjeltia, Daniel Monteyne |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lysis
medicine.medical_treatment Biophysics Context (language use) Biophysique 030204 cardiovascular system & hematology Tissue plasminogen activator Fibrin 03 medical and health sciences 0302 clinical medicine Fibrinolysis medicine Humans Biochemical reactions Patient treatment 030304 developmental biology 0303 health sciences biology Chemistry Thrombosis Clot lysis biology.protein 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Biophysical Journal Biophysical journal |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2021.08.005 |
| Popis: | It has been observed in vitro that complete clot lysis is generally preceded by a slow phase of lysis during which the degradation seems to be inefficient. However, this slow regime was merely noticed, but not yet quantitatively discussed. In our experiments, we observed that the lysis ubiquitously occurred in two distinct regimes, a slow and a fast lysis regime. We quantified extensively the duration of these regimes for a wide spectrum of experimental conditions and found that on average, the slow regime lasts longer than the fast one, meaning that during most of the process, the lysis is ineffective. We proposed a computational model in which the properties of the binding of the proteins change during the lysis: first, the biochemical reactions take place at the surface of the fibrin fibers, then in the bulk, resulting in the observed fast lysis regime. This simple hypothesis appeared to be sufficient to reproduce with a great accuracy the lysis profiles obtained experimentally. SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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