Inhibition of TYRO3/Akt signaling participates in hypoxic injury in hippocampal neurons
| Autor: | Na Xian, Bing Wu, Wei Wang, Yan-zhen Zhu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
hippocampal neurons proliferation Biology Hippocampal formation Immunofluorescence lcsh:RC346-429 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience medicine nerve regeneration TYRO3 Akt apoptosis primary culture hypoxia neural regeneration Protein kinase B lcsh:Neurology. Diseases of the nervous system medicine.diagnostic_test Akt/PKB signaling pathway Western blot assay Hypoxia (medical) Cell biology 030104 developmental biology Apoptosis medicine.symptom Neuroscience 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research, Vol 11, Iss 5, Pp 752-757 (2016) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| Popis: | In this study, we investigated the role of the TYRO3/Akt signaling pathway in hypoxic injury to hippocampal neurons. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that hypoxia inhibited the proliferation and viability of hippocampal neurons. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay demonstrated that hypoxia induced neuronal apoptosis in a time-dependent manner, with a greater number of apoptotic cells with longer hypoxic exposure. Immunofluorescence labeling revealed that hypoxia suppressed TYRO3 expression. Western blot assay showed that hypoxia decreased Akt phosphorylation levels in a time-dependent manner. Taken together, these findings suggest that hypoxia inhibits the proliferation of hippocampal neurons and promotes apoptosis, and that the inhibition of the TYRO3/Akt signaling pathway plays an important role in hypoxia-induced neuronal injury. |
| Databáze: | OpenAIRE |
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