Point-of-Care Toolkit for Multiplex Molecular Diagnosis of SARS-CoV-2 and Influenza A and B Viruses
| Autor: | A. Fatih Sarioglu, Dohwan Lee, Chia-Heng Chu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Point-of-Care Systems
viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Herpesvirus 1 Cercopithecine point-of-care RNA test Bioengineering Article influenza virus Virus Influenza Human programmed paper microfluidics Humans Medicine Multiplex Viral rna multiplexed molecular diagnosis Instrumentation Point of care Fluid Flow and Transfer Processes SARS-CoV-2 business.industry Transmission (medicine) Process Chemistry and Technology fungi COVID-19 virus diseases Influenza a Virology Mild symptoms business isothermal PCR amplification |
| Zdroj: | ACS Sensors |
| ISSN: | 2379-3694 |
| DOI: | 10.1021/acssensors.1c00702 |
| Popis: | Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is still spreading around the globe causing immense public health and socioeconomic problems. As the infection can progress with mild symptoms that can be misinterpreted as the flu, self-testing methods that can positively identify SARS-CoV-2 are needed to effectively track and prevent the transmission of the virus. In this work, we report a point-of-care toolkit for multiplex molecular diagnosis of SARS-CoV-2 and influenza A and B viruses in saliva samples. Our assay is physically programmed to run a sequence of chemical reactions on a paper substrate and internally generate heat to drive these reactions for an autonomous extraction, purification, and amplification of the viral RNA. Using our assay, we could reliably detect SARS-CoV-2 and influenza viruses at concentrations as low as 50 copies/μL visually from a colorimetric analysis. The capability to autonomously perform a traditionally labor-intensive genetic assay on a disposable platform will enable frequent, on-demand self-testing, a critical need to track and contain this and future outbreaks. |
| Databáze: | OpenAIRE |
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