Expression of tandem gene fusions in transgenic tobacco plants
| Autor: | Stanley Tamaki, Caroline Dean, Mitchell Favreau, John Bedbrook, Diane Bond-Nutter, Pamela Dunsmuir |
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| Jazyk: | angličtina |
| Rok vydání: | 1988 |
| Předmět: |
Regulation of gene expression
Genetics biology Transcription Genetic Nicotiana tabacum Transgene Genetic Vectors biology.organism_classification Molecular biology genomic DNA Plants Toxic Plasmid Gene Expression Regulation Transcription (biology) Multigene Family Gene expression Tobacco Genetic Engineering Gene Plasmids Rhizobium |
| Popis: | We have studied the expression of four sets of tandem gene fusions in transgenic tobacco plants. This was to determine if the problem of between-transformant variability in expression of introduced genes could be overcome by using a linked reference gene as a co-ordinately expressed control. Tandem gene fusions containing identical 5' flanking regions (SSU301-ocs with either SSU301-cat or SSU301-SSU911) were not co-ordinately expressed in the transgenic tobacco plants whereas the tandem gene fusions containing similar but not identical 5' flanking regions (SSU301-ocs with SSU911-cat or SSU911-SSU301) were co-ordinately expressed. The lack of co-ordinate expression of some of the tandem gene fusions appears to be partially explained by absence of the corresponding genomic DNA segments in the transgenic plants. |
| Databáze: | OpenAIRE |
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