Massilia solisilvae sp. nov., Massilia terrae sp. nov. and Massilia agilis sp. nov., isolated from forest soil in South Korea by using a newly developed culture method
| Autor: | Jaisoo Kim, Khulan Altankhuu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial Ubiquinone 030106 microbiology Forests Massilia haematophila Microbiology 03 medical and health sciences Oxalobacteraceae RNA Ribosomal 16S Republic of Korea Putrescine Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Soil Microbiology Base Composition biology Phylogenetic tree DNA–DNA hybridization Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques genomic DNA Taxonomy (biology) Proteobacteria Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 67(8) |
| ISSN: | 1466-5034 |
| Popis: | Using a newly developed culture method for not yet cultured soil bacteria, three Gram-stain-negative, aerobic, non-spore-forming, motile, and rod-shaped bacteria (strain designated J18T, J11T and J9T) were isolated from forest soil at Kyonggi University, South Korea. Isolates were subjected to a taxonomic study by using a polyphasic approach. According to a phylogenetic tree based on 16S rRNA gene sequences, strains J18T, J11T and J9T belonged to the genus Massilia and clustered with Massilia haematophila CCUG 38318T (similarity range: 97.6~98.0 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and the genomic DNA G+C contents of strains J18T, J11T and J9T were 63.4, 68.7 and 64.5 mol%, respectively. The major polyamines were putrescine and 2-hydroxyputescine, which were detected in all three strains. DNA–DNA between the three tested strains and the reference strains much lower than 70 %, the recommended threshold value for the delineation of genomic species. The predominant respiratory quinine was ubiquinone-8 (Q-8) and the major cellular fatty acids were Summed feature 3 (C16 : 1ω6c/C16 : 1ω7c) and C16 : 0. On the basis of phenotypic and genotypic data and DNA–DNA hybridization results, the three isolates are considered to represent three novel species of the genus Massilia , for which the names Massilia solisilvae sp. nov. for type strain J18T (=KEMB 9005-366T=JCM 31607T), Massilia terrae sp. nov. for type strain J11T (=KEMB 9005-360T=JCM 31606T) and Massilia agilis sp. nov. for type strain J9T (=KEMB 9005-359T=JCM 31605T) are proposed. |
| Databáze: | OpenAIRE |
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