Analysis of HrpG regulons and HrpG‐interacting proteins by ChIP‐seq and affinity proteomics in Xanthomonas campestris
| Autor: | Jin-Wei Wei, Wei Qian, Chao-Ying Deng, Hong-Yu Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hypersensitive response Crops Agricultural Proteomics ChIP Soil Science Virulence Plant Science Biology Xanthomonas campestris 01 natural sciences Regulon Type three secretion system 03 medical and health sciences Xanthomonas Bacterial Proteins Type III Secretion Systems Molecular Biology Plant Diseases Tandem affinity purification Genetics Original Articles Gene Expression Regulation Bacterial biology.organism_classification 030104 developmental biology bacteria Chromatin Immunoprecipitation Sequencing Original Article Agronomy and Crop Science Chromatin immunoprecipitation HrpG TAP 010606 plant biology & botany Genome-Wide Association Study Transcription Factors |
| Zdroj: | Molecular Plant Pathology |
| ISSN: | 1364-3703 1464-6722 |
| Popis: | Gamma‐proteobacteria Xanthomonas spp. cause at least 350 different plant diseases among important agricultural crops, which result in serious yield losses. Xanthomonas spp. rely mainly on the type III secretion system (T3SS) to infect their hosts and induce a hypersensitive response in nonhosts. HrpG, the master regulator of the T3SS, plays the dominant role in bacterial virulence. In this study, we used chromatin immunoprecipitation followed by sequencing (ChIP‐seq) and tandem affinity purification (TAP) to systematically characterize the HrpG regulon and HrpG interacting proteins in vivo. We obtained 186 candidate HrpG downstream genes from the ChIP‐seq analysis, which represented the genomic‐wide regulon spectrum. A consensus HrpG‐binding motif was obtained and three T3SS genes, hpa2, hrcU, and hrpE, were confirmed to be directly transcriptionally activated by HrpG in the inducing medium. A total of 273 putative HrpG interacting proteins were identified from the TAP data and the DNA‐binding histone‐like HU protein of Xanthomonas campestris pv. campestris (HUxcc) was proved to be involved in bacterial virulence by increasing the complexity and intelligence of the bacterial signalling pathways in the T3SS. HrpG, a global regulator, plays the central role in bacterial virulence and physiological pathways. |
| Databáze: | OpenAIRE |
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