Possible mechanisms for the renoprotective action of adipose-derived mesenchymal stem cells with CD44-targeted hyaluronic acid against renal ischemia
| Autor: | Abdelaziz M. Hussein, Eman T. Hamam, Ahmed A. Shokeir, Mohamed Ali, Amira Awadalla, Raghda W. Magar, Nashwa Barakat |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Renal function Adipose tissue Apoptosis Kidney Mesenchymal Stem Cell Transplantation 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology Nitric oxide Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ischemia Internal medicine medicine Animals General Pharmacology Toxicology and Pharmaceutics Hyaluronic Acid Inflammation Renal ischemia Mesenchymal Stem Cells General Medicine Glutathione Malondialdehyde Rats Vascular endothelial growth factor MicroRNAs Oxidative Stress 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Adipose Tissue Reperfusion Injury Kidney Diseases |
| Zdroj: | Life sciences. 272 |
| ISSN: | 1879-0631 |
| Popis: | The present study aimed to investigate the invitro preconditioning of adipose-derived mesenchymal stem cells (ADMSCs) with CD44-targeted hyalournic acid (HA) on ischemic kidney injury in rats. Ninety male Sprague Dawley rats were randomly allocated into the following groups; i) sham group, ii) control group: rats exposed to 45 min left renal ischemia with saline treatment, iii) HA group as control group but rats treated with HA, iv) ADMSCs group as control but rats treated with ADMSCs v) HA + ADMSCs group as ADMSCs but rats treated with ADMSCs preconditioned with CD44-tageted HA for 14 days. We found that treattment with either ADMSCs or HA + ADMSCs caused significant decrease in the elevated serum creatinine and BUN and malondialdehyde (MDA) concentrations and expression of TGF-β1, fibronectin, collagen type I, inducible nitric oxide synthease (iNOS) and microRNAs (miR-21, miR-17-5p, miR-10a) in kidney and significant increase in creatinine clearance, superoxide dismutase (SOD), reduced glutathione (GSH) and the expression of Bcl2, vascular endothelial growth factor (VEGF), Wnt/β-catenin pathway genes in kidney compared to control group (p 0.05). Moreover, HA + ADMSCs group caused more significant improvement in these parameters than ADMSCs group (p 0.05), while HA group did not cause any significant improvement in these parameters compared to control group. These results suggest that preconditioning of ADMSCs preconditioned with CD44-targted HA enhanced their cytoprotective effect against ischemic kidney injury. This renoprotective effect might be due to activation of angiogenesis, Wnt/β-catenin pathway proteins, and suppression of oxidative stress, apoptosis, inflammation and fibrosis. |
| Databáze: | OpenAIRE |
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