HIV infection of mononuclear cells is calcium-dependent
| Autor: | Joshua J. Anzinger, Isaac Mezo, Larry L. Thomas, Gregory T. Spear, Xin Ji, Ali M. Gabali |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
HIV Core Protein p24 Human immunodeficiency virus (HIV) Virus Attachment chemistry.chemical_element Biology Calcium medicine.disease_cause Antiviral Agents Peripheral blood mononuclear cell Virus Divalent Mannans chemistry.chemical_compound Virology medicine Humans Magnesium Trypsin Cells Cultured Edetic Acid Mannan chemistry.chemical_classification Manganese HIV virus diseases hemic and immune systems Virus Internalization Intercellular Adhesion Molecule-1 EGTA Infectious Diseases chemistry Leukocytes Mononuclear medicine.drug |
| Zdroj: | Virus Research. 122:183-188 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2006.06.006 |
| Popis: | Strategies that prevent initial HIV infection of cells are greatly needed. In this study, we determined the requirement of divalent cations for HIV infection of and attachment to peripheral blood mononuclear cells (PBMC), which contain several types of HIV-infectable cells—CD4+ T cells, monocytes and dendritic cells. EDTA, added only during PBMC exposure to HIV, reduced infection by an average of 92%. The reduction of infection by EDTA was accompanied by a reduction in HIV binding to PBMC; R5, X4 and dual-tropic HIV binding to PBMC were inhibited by >85%. EGTA similarly reduced HIV binding to PBMC, while addition of Ca2+ or Mn2+, but not Mg2+, fully restored binding. Virus attachment was inhibited in a dose-dependent manner by trypsin treatment of PBMC, indicating protein involvement in HIV binding. In contrast, mannan or soluble ICAM-1 did not inhibit HIV binding to PBMC. These data indicate that a Ca2+-dependent cell-surface protein(s) is responsible for the majority of HIV attachment to and infection of PBMC. Further studies of this are likely to reveal novel strategies to prevent infection of PBMC. |
| Databáze: | OpenAIRE |
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