Superparamagnetic iron oxide particles transactivator protein-fluorescein isothiocyanate particle labeling for in vivo magnetic resonance imaging detection of cell migration: uptake and durability
| Autor: | Michael K. Tanner, Traci L. Smith, L. Madison Ryle, Mangay Williams, Christina L. Kaufman, Chien Ho |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Iron Cell Mice Inbred Strains Mice chemistry.chemical_compound Drug Stability Cell Movement medicine Animals Fluorescein Magnetite Nanoparticles Fluorescein isothiocyanate Transplantation Chemistry business.industry Dextrans Oxides Rats Inbred Strains Cell migration Magnetic Resonance Imaging Ferrosoferric Oxide Rats Cross-Linking Reagents Gene Products rev medicine.anatomical_structure Cytoplasm Isothiocyanate Biophysics Particle Female Nuclear medicine business Fluorescein-5-isothiocyanate Spleen |
| Zdroj: | Transplantation. 76:1043-1046 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/01.tp.0000090164.42732.47 |
| Popis: | Conjugation of dextran-coated superparamagnetic iron oxide (SPIO) particles with transactivator protein (Tat)-peptide and fluorescein isothiocyanate (FITC) allows cells to readily uptake SPIO particles. This makes possible high-resolution, real-time imaging of these cells by magnetic resonance imaging (MRI). First, we need to understand how various subpopulations take up and maintain SPIO particles. In this report, we have focused on differences in T cells, B cells, and macrophages with respect to cross-linked (CL)-SPIO Tat-FITC particle uptake over 72 hours. We have found that cells quickly take up the particles and that the bead loss that does occur is not related to cell death or apoptosis. In contrast with reports in the literature, we have observed migration of the Tat-peptide conjugates primarily to the cytoplasm rather than the nucleus. |
| Databáze: | OpenAIRE |
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