Saccharedens versatilis gen. nov., sp. nov., a sugar-degrading member of the Burkholderiales isolated from Cephalotes rohweri ant guts
| Autor: | John T. Wertz, William J. Hobson, Jonathan Y. Lin |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Ubiquinone Cephalotes Microbiology 03 medical and health sciences chemistry.chemical_compound Phylogenetics RNA Ribosomal 16S Botany Animals Trypticase soy agar Symbiosis Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Base Composition biology Strain (chemistry) Ants Fatty Acids Arizona Sequence Analysis DNA General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Burkholderiales 030104 developmental biology chemistry lipids (amino acids peptides and proteins) Bordetella petrii Alcaligenaceae |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 67:447-453 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001650 |
| Popis: | Cephalotes ‘turtle’ ants host a core group of gut–associated symbionts, but their potential contributions to ant nutrition and disease resistance remain uncharacterized in vitro. To gain a better understanding of the metabolic capability of core symbionts belonging to the Burkholderiales , we cultivated and characterized strain CAG32T from the guts of Cephalotes rohweri ants. Strain CAG32T was rod-shaped, Gram-stain-negative, motile and formed pale-white colonies on trypticase soy agar. Optimum growth occurred under an atmosphere of 20 % O2 supplemented with 1 % CO2. Strain CAG32T grew under NaCl concentrations of 0–2.0 %, temperatures of 23–47 °C and pH values of 4.0–8.0, and was capable of producing n-butyric acid and degrading carbohydrates for growth. The G+C content of the genomic DNA was 59.2±0.6 mol% and the major fatty acids were C16 : 0, C16 : 1 ω7c/C16 : 1 ω6c, C17 : 0 cylcopropane, C12 : 0 and C14 : 0 3-OH/C16 : 1 iso I. The only respiratory quinone detected was ubiquinone-8 (Q-8) and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on phylogenetic analysis of the 16S rRNA gene sequence, strain CAG32T shared 96.9 % nucleotide similarity with its closest cultivated neighbours Bordetella petrii Se-1111RT and Bordetella bronchiseptica ATCC 19395T. This, combined with differences in the phenotypic and biochemical profile from neighbouring strains, warrants the classification of strain CAG32T as representing a novel species of a new genus within the Burkholderiales family Alcaligenaceae . The name Saccharedens versatilis gen. nov., sp. nov. is proposed. The type strain of Saccharedens versatilis is CAG32T (=NCIMB 15010T=DSM 100909T). |
| Databáze: | OpenAIRE |
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