Adaptation of a trap door technique for the recovery of ovarian cortical biopsies from Cebus apella (capuchin monkey)
| Autor: | Julianne S. Lima, Regiane R. Santos, Luana N. S. Santana, Sheyla Farhayldes Souza Domingues, Adriel B. Brito, Danielle C. Brito |
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| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Biopsy Ovarian biopsy Biology Ovarian structure Cryopreservation Ovarian Follicle Follicular phase medicine Animals Cebus Humans Microscopy Ultrasonography Doppler Duplex medicine.diagnostic_test business.industry Ovarian tissue Ultrasound Trap door Cell Biology Anatomy Plastic Surgery Procedures Female business Developmental Biology |
| Zdroj: | Zygote (Cambridge, England). 21(2) |
| ISSN: | 1469-8730 |
| Popis: | SummaryThere is a paucity of efficient cryopreservation protocols for primordial follicles enclosed in the ovarian tissue from non-human primates (NHP), in special New World primates. Our objective was to establish an optimal procedure for the recovery of ovarian biopsies from capuchin monkeys. To this end, we adapted a trap door biopsy method. Follicular density and quality of the biopsies were evaluated and ultrasound analysis was performed before and continuously after surgery to assess ovarian structure. Ovarian tissue biopsies recovered by the trap door technique allowed the successful harvesting of primordial follicles from capuchin monkeys, and no complication was recorded. The female cycle was not affected by surgery and no adherence was found thereafter. In conclusion, the adaptation of a trap door biopsy method is a safe procedure and allows recovery of healthy primordial follicles. |
| Databáze: | OpenAIRE |
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