Phenol red: an inhibitor of thyroglobulin iodination in cultured porcine thyroid cells
| Autor: | D. Gruffat, S. Gonzalvez, Jean Mauchamp, Odile Chabaud |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Swine
medicine.medical_treatment Thyroid Gland chemistry.chemical_element Iodine Biochemistry Thyroglobulin Epithelium Phenolsulfonphthalein Tissue culture chemistry.chemical_compound Endocrinology Thyroid peroxidase medicine Animals Molecular Biology Cells Cultured Peroxidase Phenol red biology Thyroid Substrate (chemistry) Cell Polarity Epithelial Cells Iodides Molecular biology medicine.anatomical_structure chemistry Cell culture biology.protein |
| Zdroj: | Molecular and cellular endocrinology. 81(1-3) |
| ISSN: | 0303-7207 |
| Popis: | Phenol red, commonly used as a pH indicator in tissue culture media, is known to possess estrogenic properties. We investigated the effect of phenol red on the process of thyroglobulin iodination which occurs only at the apical surface of porcine thyroid cells when cultured in porous bottom chambers. When phenol red was added simultaneously to both compartments (apical and basolateral), separated by the polarized monolayer, thyroglobulin iodination was inhibited by about 86% without any effect on thyroglobulin secretion and apical iodine concentration. When phenol red was added separately to either the apical or basal media, inhibition was 68% and 43%, respectively. A large amount of phenol red which was introduced into the basal medium crossed through the monolayer. Thus, inhibition was dependent upon the concentration of phenol red present in the apical compartment. A maximal inhibition was observed from 30 μM apical concentration. Phenol red acts as a substrate for thyroperoxidase in the iodination reaction. |
| Databáze: | OpenAIRE |
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