Isolation of lipid- and polysaccharide-degrading micro-organisms from subtropical forest soil, and analysis of lipolytic strain Bacillus sp. CR-179

Autor: Cristian Ruiz, F.I.J. Pastor, Pilar Diaz
Rok vydání: 2005
Předmět:
Zdroj: Letters in Applied Microbiology. 40:218-227
ISSN: 0266-8254
DOI: 10.1111/j.1472-765x.2005.01660.x
Popis: C. RUIZ, F.I.J. PASTOR AND P. DIAZ.2005. Aims: To isolate the micro-organisms from three soil samples obtained from a subtropical forest of Puerto Iguazu ´ (Argentina), to analyse them for detection of the biotechnologically interesting enzymatic activities lipase, esterase, cellulase, xylanase and pectinase, and to identify the most active strain. Methods and Results: A total of 724 strains were isolated using different culture media and temperatures, and 449 of them showed at least one of the hydrolytic activities pursued. Lipolytic activity of the lipid-degrading strains was further determined using MUF-butyrate and MUF-oleate as substrates. The alkalophilic strain CR-179, one of the most active for all the enzymatic activities assayed, was characterized and preliminarily identified by morphological, physiological and 16S rDNA tests, as a Bacillus sp. closely related to Bacillus subtilis. Conclusions: Highly hydrolytic strains were isolated from all soil samples, suggesting the existence of a microbial community well-adapted to nutrient recycling. Strain CR-179, one of the most active, has been preliminarily identified as a Bacillus sp. Significance and Impact of the Study: A collection of hydrolytic strains with high biotechnological potential was obtained. Presence of sequences codifying for a lipolytic system related to the B. subtilis group lipases was revealed by PCR for the best lipolytic strain.
Databáze: OpenAIRE
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