Calnexin functions in antibacterial immunity of Marsupenaeus japonicus
| Autor: | Xiao-Fan Zhao, Jin-Xing Wang, Wen-Ming Jia, Qing Zhang, Hai-Shan Jiang, Xiu-Qing Wang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Staphylococcus aureus
Hemocytes Calnexin Phagocytosis Immunology Gene Expression Bacillus Biology Microbiology Arthropod Proteins Micrococcus Penaeidae RNA interference Animals Cells Cultured Phylogeny Vibrio Innate immune system Endoplasmic reticulum Polysaccharides Bacterial Pattern recognition receptor Immunity Innate Cell biology Protein Transport Chaperone (protein) biology.protein Calreticulin Developmental Biology Protein Binding |
| Zdroj: | Developmental and comparative immunology. 46(2) |
| ISSN: | 1879-0089 |
| Popis: | Calnexin (Cnx) is an endoplasmic reticulum membrane–bound lectin chaperone that comprises a dedicated maturation system with another lectin chaperone calreticulin (Crt). This maturation system is known as the Cnx/Crt cycle. The main functions of Cnx are Ca 2+ storage, glycoprotein folding, and quality control of synthesis. Recent studies have shown that Cnx is important in phagocytosis and in optimizing dendritic cell immunity. However, the functions of Cnx in invertebrate innate immunity remain unclear. In this research, we characterized Cnx in the kuruma shrimp Marsupenaeus japonicus (designated as Mj Cnx) and detected its function in shrimp immunity. The expression of MjCnx was upregulated in several tissues challenged with Vibrio anguillarum . Recombinant Mj Cnx could bind to bacteria by binding polysaccharides. Mj Cnx protein existed in the cytoplasm and on the membrane of hemocytes and was upregulated by bacterial challenge. The recombinant Mj Cnx enhanced the clearance of V. anguillarum in vivo, and the clearance effects were impaired after silencing MjCnx with RNA interference assay. Recombinant Mj Cnx promoted phagocytosis efficiency of hemocytes. These results suggest that Mj Cnx functions as one of the pattern recognition receptors and has crucial functions in shrimp antibacterial immunity. |
| Databáze: | OpenAIRE |
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