Search for retrovirus in the chronic fatigue syndrome
Autor: | K. Simpson, Lindsay Morrison, Peter O. Behan, Heather Cavanagh, Axel Rethwilm, W. M. H. Behan, John W. Gow, Andreas Schliephake |
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Rok vydání: | 1992 |
Předmět: |
Virologie
viruses Blotting Western Polymerase Chain Reaction Virus Pathology and Forensic Medicine law.invention Retrovirus law Chronic fatigue syndrome medicine Humans ddc:610 Polymerase chain reaction Human T-lymphotropic virus 1 Fatigue Syndrome Chronic biology Muscles Human T-lymphotropic virus 2 General Medicine Human foamy virus biology.organism_classification medicine.disease Virology Blotting Southern Immunology biology.protein Antibody Research Article |
Zdroj: | Scopus-Elsevier |
ISSN: | 0021-9746 |
Popis: | AIM: To examine peripheral blood and skeletal muscle from patients with chronic fatigue syndrome for exogenous retrovirus. METHODS: Blood samples from 30 patients and muscle biopsy specimens of 15 patients were examined for retroviral sequences by DNA extraction, polymerase chain reaction (PCR), and Southern blotting hybridisation. Sera were examined for human foamy virus by western immunoblotting and indirect immunofluorescence techniques. RESULTS: No differences between the patient and control populations was found for any of the PCR primer sets used (gag, pol, env, and tax regions of HTLV I/II). An endogenous gag band was observed in both the patient and control groups. All sera were negative for antibody to human foamy virus. CONCLUSION: The results indicate that there is no evidence of retroviral involvement in the chronic fatigue syndrome. |
Databáze: | OpenAIRE |
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