Cloning of the riboB locus of Aspergillus nidulans
| Autor: | Berl R. Oakley, Kretz Pl, Clifford F. Weil, Oakley Ce |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Recombination
Genetic Genetics biology Riboflavin Genes Fungal Genetic Complementation Test Genetic transfer Chromosome Mapping Locus (genetics) DNA Restriction Enzymes General Medicine Molecular cloning biology.organism_classification Molecular biology Aspergillus nidulans Complementation Transformation (genetics) Plasmid Genes Mutation Cloning Molecular Homologous recombination |
| Zdroj: | Gene. 53:293-298 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(87)90019-9 |
| Popis: | We have complemented the riboB2 mutation of Aspergillus nidulans by transformation with a plasmid library of wild-type (wt) sequences. We have isolated, by marker rescue from a riboB+ transformant, a plasmid that complements riboB2 efficiently. From this plasmid we have subcloned an A. nidulans sequence that complements riboB2 efficiently and that integrates by homologous recombination at a site closely linked to the riboB locus. We conclude that this sequence contains the wt riboB+ allele. |
| Databáze: | OpenAIRE |
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