Polymorphonuclear leukocyte accumulation in brain regions with low blood flow during the early postischemic period
| Autor: | Andrew J. Dutka, C B Thompson, T J Contreras, T P Obrenovitch, John M. Hallenbeck, Patrick M. Kochanek, T. Tanishima, K K Kumaroo |
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| Rok vydání: | 1986 |
| Předmět: |
Male
medicine.medical_specialty Neutrophils Period (gene) Ischemia Blood Pressure Granulocyte Air embolism Brain Ischemia Dogs Gamma scintigraphy Heart Rate Evoked Potentials Somatosensory Internal medicine medicine Animals Advanced and Specialized Nursing Polymorphonuclear leukocyte Analysis of Variance business.industry Brain Blood flow Hydrogen-Ion Concentration medicine.disease medicine.anatomical_structure Endocrinology Hematocrit Regional Blood Flow Cerebrovascular Circulation Anesthesia Autoradiography Neurology (clinical) Blood Gas Analysis Cardiology and Cardiovascular Medicine business Canine model Mathematics Granulocytes |
| Zdroj: | Stroke. 17:246-253 |
| ISSN: | 1524-4628 0039-2499 |
| DOI: | 10.1161/01.str.17.2.246 |
| Popis: | In an anesthetized canine model in which ischemia was induced by incremental air embolism, 16 animals were exposed to 1 hr of ischemia and monitored for 10 min (n = 4), 60 min (n = 6), or 240 min (n = 6). Fourteen animals were observed for corresponding periods without being subjected to ischemia 70 min (n = 4), 120 min (n = 4), or 300 min (n = 6). Autologous granulocytes were labeled with 111In and reinfused just before ischemia. At the conclusion of each experiment, a 14C-iodoantipyrine autoradiographic blood flow study was performed. Granulocyte accumulation measured by gamma scintigraphy (cpm/gm) occurred in the injured hemisphere of ischemic animals at 60 min in anterior brain segments and at 240 min in anterior, middle, and posterior segments. By means of a double-label autoradiography technique, clustering of punctate granulocyte images was detected in regions of low flow or heterogeneous flow in half of the animals at both 60 min and 240 min postischemia. Granulocyte clustering did not occur in the autoradiograms of nonischemic animals. The results implicate granulocyte participation in the acute phase of ischemic brain injury and signal a convergence of hemostatic and inflammatory processes during the immediate postischemic period. |
| Databáze: | OpenAIRE |
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