LC-MS/MS Determination of Mono-Glutamate Folates and Folic Acid in Beer
| Autor: | Terenzio Bertuzzi, Annalisa Mulazzi, Silvia Rastelli, Filippo Rossi |
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| Rok vydání: | 2018 |
| Předmět: |
Detection limit
Chromatography Chemistry 010401 analytical chemistry Glutamate receptor 04 agricultural and veterinary sciences folates 040401 food science 01 natural sciences Applied Microbiology and Biotechnology 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Settore AGR/15 - SCIENZE E TECNOLOGIE ALIMENTARI 0404 agricultural biotechnology Folic acid Lc ms ms Vitamer Methanol Safety Risk Reliability and Quality Acetonitrile Safety Research Food Science |
| Zdroj: | Università Cattolica del Sacro Cuore-IRIS |
| ISSN: | 1936-976X 1936-9751 |
| DOI: | 10.1007/s12161-018-1396-6 |
| Popis: | A reliable LC-MS/MS method was developed for determination of folates in beer. Clean-up steps and chromatographic conditions were optimized to remove interferences and decrease the matrix effect. The work showed that an immuno-affinity column designed for folic acid (FA) was also suitable for the oxidized forms 10-formylfolic acid (10-HCO-FA) and 7,8-dihydrofolic acid (DHF), obtaining clean extract and improving the limit of detection. For the other vitamer forms, an Oasis MAX column was used. For chromatographic conditions, a RP-18 column with low ligand density showed satisfactory retention times; moreover, the use of methanol instead of acetonitrile lowered the limit of detection up to 0.1 μg l−1. The most popular beers in Italy were collected and analyzed; mainly oxidized mono-glutamate forms, in particular 10-HCO-FA and FA, were found, at levels from 12.4 to 98.6 μg l−1 and 1.4 and 11.9 μg l−1, respectively. The vitamers 5-formyl-tetrahydrofolate and 5-methyl-tetrahydrofolate were also detected. |
| Databáze: | OpenAIRE |
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