A carbon nanotube-based Raman-imaging immunoassay for evaluating tumor targeting ligands
| Autor: | Paul Pantano, Pauras Memon, Ruhung Wang, Gregg R. Dieckmann, Pooja Bajaj, David Bushdiecker, Inga H. Musselman, Carole Mikoryak, Rockford K. Draper |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.drug_class
Confocal Fluorescent Antibody Technique Monoclonal antibody Ligands Microscopy Atomic Force Biochemistry Analytical Chemistry symbols.namesake Microscopy Electron Transmission Cell surface receptor Cell Line Tumor Neoplasms Microscopy Electrochemistry medicine Environmental Chemistry Humans Receptor Spectroscopy Immunoassay medicine.diagnostic_test Chemistry Nanotubes Carbon Spectrum Analysis Molecular biology Biotinylation symbols Biophysics Raman spectroscopy |
| Zdroj: | The Analyst. 139(12) |
| ISSN: | 1364-5528 |
| Popis: | Herein, we describe a versatile immunoassay that uses biotinylated single-walled carbon nanotubes (SWNTs) as a Raman label, avidin–biotin chemistry to link targeting ligands to the label, and confocal Raman microscopy to image whole cells. Using a breast tumor cell model, we demonstrate the usefulness of the method to assess membrane receptor/ligand systems by evaluating a monoclonal antibody, Her-66, known to target the Her2 receptors that are overexpressed on these cells. We present two-dimensional Raman images of the cellular distribution of the SWNT labels corresponding to the distribution of the Her2 receptors in different focal planes through the cell with validation of the method using immunofluorescence microscopy, demonstrating that the Her-66–SWNT complexes were targeted to Her2 cell receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|