Dynactin pathway-related gene expression is altered by aging, but not by vitrification
| Autor: | Sara Franchi, Liborio Stuppia, Marco D'Aurora, Annalina Sarra, Maria Cristina Budani, Valentina Gatta, Gian Mario Tiboni |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Aging Reproductive Techniques Assisted medicine.medical_treatment Gene Expression Oocyte Retrieval Aneuploidy 010501 environmental sciences Biology Real-Time Polymerase Chain Reaction Toxicology 01 natural sciences Cryopreservation 03 medical and health sciences Meiosis medicine Humans 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Assisted reproductive technology Age Factors Dynactin Complex Oocyte medicine.disease Vitrification Cell biology DCTN1 medicine.anatomical_structure Gene Expression Regulation Ageing Oocytes Dynactin |
| Zdroj: | Reproductive Toxicology. 88:48-55 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2019.06.011 |
| Popis: | The storage of surplus oocytes by cryopreservation (OC) is a widely used tool in assisted reproductive technology, but there is a great debate about the effects of cryopreservation on oocyte competence. It is known that OC may affect meiotic spindles but remains unclear if OC may increase the risk of aneuploidy. The aim of this study was to test the effects of OC and women aging on the expression of cytokinesis-related genes playing an important role in the segregation of chromosomes (DCTN1, DCTN2, DCTN3, DCTN6 and PLK1). Results highlighted that OC do not modify the expression of the selected genes, whereas women aging modulate the expression of all transcripts, confirming that aging is the crucial factor affecting meiosis and aneuploidy risk. A new role for Dynactin and PLK1 was shed in light, providing information on the ageing process in the oocyte which may be associated to reduced fertility. |
| Databáze: | OpenAIRE |
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